Fig. 4
From: CAF secreted miR-522 suppresses ferroptosis and promotes acquired chemo-resistance in gastric cancer
USP7 promotes miR-522 secretion by regulating deubiquitination of hnRNPA1 in CAFs. a. IHC analysis of USP7, hnRNPA1 and ALOX15 by using serial sections in paired para-carcinoma tissues and tumor tissues (n = 12). b. WB analysis of hnRNPA1 and USP7 in 12 paired CAFs and NFs (n = 12). c-d. Quantitative analysis of hnRNPA1 (c) and USP7 (d) described in (b) (n = 12). e-g. Analysis of the correlation between USP7, hnRNPA1 and exo-miR-522 in CAFs. HnRNPA1 is positively related with exo-miR-522 (e), and USP7 also showed positive relevance with both exo-miR-522 (f) and hnRNPA1 (g) (n = 12). h. WB analysis of hnRNPA1 and USP7 in CAFs treated with hnRNPA1 overexpression plasmids (OE.hnRNPA1), hnRNPA1 siRNA (si.hnRNPA1), USP7 overexpression plasmids (OE.USP7) and USP7 siRNA (si. USP7) respectively (n = 3). i. Quantitative analysis of (g) (n = 12). j. USP7/hnRNPA1 promotes miR-522 secretion (n = 3). k. Immuno-precipitation shows the direct interaction between USP7 and hnRNPA1 in CAFs (n = 3). l. WB analysis of hnRNPA1 ubiquitination in CAFs treated with OE.USP7 or si. USP7 (n = 3). ** indicates p < 0.01