Fig. 5

Direct evidence for hnRNPA1 mediated miR-522 packaging into CAF exosomes. a. RBPDB analysis of the specific interaction between miR-522 and RBP motifs (threshold 0.5). b. WB analysis of hnRNPA1, PABPC1 and ACO1 expression in CAFs transfected with corresponding siRNAs (n = 3). c. Relative levels of miR-522 in CAFs transfected with siRNAs (n = 3). d. Quantification of miR-522 in CAF exosomes treated as described above (n = 3). e. Detection of hnRNPA1 protein in the samples derived from miR-522 pull downs performed in CAFs and CAF exosomes (n = 3). f. Schematic diagram for the cell co-culture model of CAFs and SGC7901 cells. h. Capture of CAF-exosome delivered Cys-miR-522 by SGC7901 cells co-cultured with CAFs (n = 3). i. Quantitative analysis of (h) (n = 3). j. Capture of CAF-exosome delivered Cys-miR-522 by MKN45 cells co-cultured with CAFs (n = 3). k. Quantitative analysis of (J) (n = 3). ** indicates p < 0.01; *** indicates p < 0.001