Fig. 7
From: CAF secreted miR-522 suppresses ferroptosis and promotes acquired chemo-resistance in gastric cancer
In vivo role of USP7/hnRNPA1/exo-miR-522 pathway in regulating ferroptosis and chemo-sensitivity of gastric tumors. a. Schematic description of the experimental design used to establish the animal model. b. Images of tumors in each group (n = 6). c. Alterations of tumor diameters in each group (n = 6). d. Weight measurements of the tumors described above (n = 6). e. Relative levels of lipid-ROS in tumors (n = 6). f. WB analysis of USP7 and hnRNPA1 in primary CAFs and ALOX15 in tumor tissues (n = 6). g. Quantitative analysis of (f). h. IHC analysis of ALOX15 distribution in tumor tissues (n = 6). i. Relative levels of ALOX15 in tumor tissues (n = 6). j. TEM images of exosomes isolated from mouse serum. k. Relative levels of miR-522 in serum exosomes (n = 6). l-m. Quantification of ferroptosis marker (l) and apoptosis marker (m) (n = 6). ** indicates p < 0.01