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Fig. 4 | Molecular Cancer

Fig. 4

From: m6A regulator-mediated methylation modification patterns and tumor microenvironment infiltration characterization in gastric cancer

Fig. 4

Construction of m6A signatures. a Unsupervised clustering of overlapping m6A phenotype-related genes in ACRG cohorts to classify patients into different genomic subtypes, termed as m6A gene cluster A-C, respectively. The gene clusters, m6Aclusters, ACRG molecular subtypes, tumor stage, histology, survival status and age were used as patient annotations. b Kaplan-Meier curves indicated m6A modification genomic phenotypes were markedly related to overall survival of 300 patients in ACRG cohort, of which 88 cases were in gene cluster A, 107 cases in gene cluster B and 105 cases in gene cluster C (P < 0.0001, Log-rank test). c The expression of 21 m6A regulators in three gene cluster. The upper and lower ends of the boxes represented interquartile range of values. The lines in the boxes represented median value, and black dots showed outliers. The asterisks represented the statistical p value (*P < 0.05; **P < 0.01; ***P < 0.001). The one-way ANOVA test was used to test the statistical differences among three gene clusters. d Alluvial diagram showing the changes of m6Aclusters, ACRG molecular subtypes, gene cluster and m6Ascore. e Correlations between m6Ascore and the known gene signatures in ACRG cohort using Spearman analysis. Negative correlation was marked with blue and positive correlation with orange. f Differences in m6Ascore among three gene clusters in ACRG cohort. The Kruskal-Wallis test was used to compare the statistical difference between three gene clusters (P < 0.001). g Differences in m6Ascore among three m6A modification patterns in ACRG cohort (P < 0.001, Kruskal-Wallis test). h Differences in stroma-activated pathways between high m6Ascore and low m6Ascore groups. APM, antigen processing machinery; EMT, epithelial-mesenchymal transition; TGFb, transforming growth factor beta. The upper and lower ends of the boxes represented interquartile range of values. The lines in the boxes represented median value. The asterisks represented the statistical p value (*P < 0.05; **P < 0.01; ***P < 0.001)

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