Fig. 5
Characteristics of m6A modification in TCGA molecular subtypes and tumor somatic mutation. a Differences in m6Ascore between different ACRG molecular subtypes. The Kruskal-Wallis test was used to compare the statistical difference between four ACRG molecular subtypes (p < 0.0001). b Survival analyses for low (157 cases) and high (143 cases) m6Ascore patient groups in ACRG cohort using Kaplan-Meier curves (HR, 3.0 (2.12–4.21); P < 0.0001, Log-rank test). c Survival analyses for subgroup patients stratified by both m6Ascore and treatment with adjuvant chemotherapy using Kaplan-Meier curves. H, high; L, Low; ADJC, adjuvant chemotherap (P < 0.0001, Log-rank test). d Survival analyses for low (157 cases) and high (143 cases) m6Ascore patient groups in the TCGA-STAD cohort using Kaplan-Meier curves (HR, 1.81(1.26–2.62); P = 0.001, Log-rank test). e Differences in m6Ascore between different TCGA-STAD molecular subtypes. The upper and lower ends of the boxes represented interquartile range of values. The lines in the boxes represented median value. The Kruskal-Wallis test was used to compare the statistical difference between four TCGA-STAD molecular subtypes (p < 0.0001). GS, genome stable; MSI, microsatellite instability; EBV, EBV infection; CIN, chromosomal instability. f Differences in m6Ascore among different of microsatellite subtypes. The upper and lower ends of the boxes represented interquartile range of values. The lines in the boxes represented median value. The asterisks represented the statistical p value (*P < 0.05; **P < 0.01; ***P < 0.001). MSS, microsatellite stable; MSI-H, high microsatellite instability; MSI-L, low microsatellite instability. g, h The waterfall plot of tumor somatic mutation established by those with high m6Ascore (g) and low m6Ascore (h). Each column represented individual patients. The upper barplot showed TMB, The number on the right indicated the mutation frequency in each gene. The right barplot showed the proportion of each variant type