Fig. 2
From: Verification of EZH2 as a druggable target in metastatic uveal melanoma
Pharmacologic inhibition of EZH2 prohibits growth of UM cells. a Targeting EZH2 by HMT inhibitors suppressed proliferation. UM cells were exposed to various concentrations of inhibitors for 72 h. Cell viability was determined by MTS assay. b-c HMT inhibitors eliminated H3K27me3 in UM. UM cells were treated with HMT inhibitors (μmol/L) as indicated for 72 h, and then subjected to Western blotting analysis. The expression levels of H3K27me3 in HMT inhibitor-treated cells comparing to those of control ones were analyzed by Image J (b). Plots were analyzed by IC50 values of HMT inhibitors in downregulating H3K27me3 expression against those in inhibiting cell viabilities of UM cells. The dots along X and Y axis represented treatment with GSK126, UNC1999, GSK503, CPI1205, EI1 and EPZ6438, respectively (c)