Fig. 6

Depletion of EZH2 abrogates the liver metastasis of UM in mice. a-c Silencing of EZH2 attenuated liver metastasis of UM in NOG mice. Mel270-luc and Omm2.3-luc cells transduced with Scramble or lentiviral shEZH2 were intrasplenically injected into NOG mice. IVIS signals of luciferase activity of Mel270-luc on day 0 and day 28 were shown (a). The curves were IVIS signals against days after inoculation (b). *, P < 0.05; **, P < 0.01, one-way ANOVA, post hoc intergroup comparisons. Photos of livers fixed in Bouin’s solution were shown (c, left) and numbers of nodules on liver surface were counted (c, middle and right). Each dot represents one mouse. **, P < 0.01; ***, P < 0.0001, one-way ANOVA, post hoc intergroup comparisons. d-g Pharmacologic inhibition of EZH2 attenuated the metastasis of UM cells. NOG mice were intrasplenically injected with Mel270-luc or Omm2.3-Luc cells and administrated with GSK126 (50 mg/kg/d, i.p.) for 4 weeks (d). The IVIS signals were recorded (e). *, P < 0.05; Student’s t test. The metastatic tumor nodules on the liver surface were counted (f). **, P < 0.01; ***, P < 0.0001, Student’s t test. IHC staining of RhoGDIγ and H3K27me3 in metastatic liver sections from Mel270-luc-bearing NOG mice after treatment of placebo or GSK126 (g). Photos were recorded by Olympus IX71. Original magnification was 100× (Scale bar, 100 μm) and 200× (Scale bar, 50 μm). h A proposed model of EZH2 in orchestrating the metastasis of UM