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Fig. 3 | Molecular Cancer

Fig. 3

From: Dying tumor cell-derived exosomal miR-194-5p potentiates survival and repopulation of tumor repopulating cells upon radiotherapy in pancreatic cancer

Fig. 3

Exosomes potentiate survival of TRCs via promoting DDR. a Representative images of H&E staining of the PDX tissues that were untreated, or treated with GW4968, radiation or radiation plus GW4869. Arrow indicates the multinuclear giant cell. Scale bar: 20 μm. b Representative images (left) and quantifications (right) of immunofluorescent staining of ALDH1A1 (red) and γH2A.X (green) in PDX tumor tissues. Tumors were treated with radiation or radiation plus GW4869. Scale bar: 50 μm. c Representative images (left) and quantifications (right) of immunofluorescent staining of GFP (green) and γH2A.X (red) in the tracing PANC-1 cells. Cells were treated with radiation or radiation plus GW4869. Scale bar: 50 μm. d Western blot detection of pATM and γH2A.X in PANC-1 cells. Cells were treated with exosomes from irradiated or unirradiated cells, and subjected to 2Gy radiation. Numbers at the bottom of corresponding bands show a ratio of γH2A.X or pATM levels relative to time 0 after normalization to GAPDH. e Representative images (left) and quantifications (right) of comet assay in PANC-1 cells. Cells were treated as in Fig. 3d. Data are presented as mean with SD of at least three independent experiments; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; NS, not significant from unpaired Student’s t test

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