Fig. 3

circSEPT9 is up-regulated in TNBC and related with progression and poor prognosis of TNBC patients. a and b The relative expression of circSEPT9 in TNBC cells and 60 paired TNBC tissues and adjacent normal tissues by qRT-PCR. c ROC curve was applied to evaluate the diagnostic value of circSEPT9 for TNBC. d Representative images showed the expression of circSEPT9 in 80 TNBC tissues detected by in situ hybridization. Scale bar, 50 μm. e The ISH staining scores were calculated in 80 TNBC tissues. ISH staining score < 6 was defined as low expression, while a score ≥ 6 was regards as high expression. f Dot distribution graph of circSEPT9 IHC staining scores was shown in 80 TNBC patients of different clinical stages. g Kaplan-Meier survival curve analysis showed that the total survival time of TNBC patients with high expression of circSEPT9 was significantly shorter than that of TNBC patients with low expression of circSEPT9. The total survival was significantly correlated with TNM stage and lymph node metastasis, but not with age, histological grade and tumor size. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001