Fig. 4

Silencing of circSEPT9 decreases proliferation, migration and invasion of TNBC cells. a The schematic illustration of circSEPT9 overexpression vector and si-circSEPT9 was shown. b-d Relative expression of circSEPT9 and SEPT9 mRNA was measured by qRT-PCR in TNBC cells transfected with circSEPT9 overexpression plasmid and si-circSEPT9. e Cell proliferation ability was evaluated by colony formation. f EdU assay of TNBC cells was performed to evaluate cell proliferation (magnification, × 100, Scale bar, 100 μm). g The growth curves of cells were measured by using CCK-8 assay. h Transwell invasion assay were used to assess the invasion abilities of TNBC cells (magnification, × 100, Scale bar, 100 μm). i and j The migration abilities of TNBC cells were measured by transwell migration (magnification, × 100, Scale bar, 100 μm) and wound healing (magnification, × 50, Scale bar, 100 μm) assays. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001