Fig. 6

circSEPT9 functions as a sponge for miR-637. a The relative expression of miR-637 was determined in TNBC cells after transfection with circSEPT9 expression vector and si-circSEPT9 by qRT-PCR. b The relative expression of miR-637 was detected in 60 paired TNBC tissues and adjacent normal tissues by qRT-PCR. c An inverse correlation was shown between miR-637 and circSEPT9 expression in 60 TNBC tissues using pearson correlation analysis. d Schematic diagram of circSEPT9 luciferase reporter vectors carrying wild-type (WT) or mutant (Mut) miR-637 binding sites was displayed. e The relative luciferase activities were measured in 293 T cells co-transfected with circSEPT9-WT or circSEPT9-Mut and miR-637 mimics or miR-NC by luciferase reporter assay. f The co-localization of circSEPT9 and miR-637 was observed in TNBC tissues (magnification, × 100, Scale bar, 50 μm) and cells (magnification, × 200, Scale bar, 50 μm) by FISH assay. g RNA pull-down with a biotin-labeled circSEPT9 probe was implemented in MDA-MB-231 cells, followed by qRT-PCR and RT-PCR to test the enrichment of circSEPT9 and miR-637. h RNA pull-down with a biotin-labeled miR-637 probe was conducted in MDA-MB-231 cells, the content of circSEPT9 was detected by qRT-PCR and RT-PCR. The data are presented as the mean ± SD, **P < 0.01, ***P < 0.001