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Fig. 5 | Molecular Cancer

Fig. 5

From: CircLONP2 enhances colorectal carcinoma invasion and metastasis through modulating the maturation and exosomal dissemination of microRNA-17

Fig. 5

circLONP2 directly interacts with DDX1. a Different protein bands detected by silver stain for mass spectrometry. b KEGG analysis of potential proteins interacting with circLONP2. c The specific amino acid sequence of DDX1 showed by second-order mass spectrum. d RNA pulldown followed by WB confirmed the interaction of circLONP2 with DDX1, DDX5 and DDX17. e, f A series of deletion mutants of DDX1 labeled with FLAG and confirmed by WB. g Anti-FLAG RIP assay showed significant enrichment of circLONP2 in SPRY domain and the full length of DDX1. h, i Rescue experiments indicated that DDX1 was essential for circLONP2-enhanced migrating ability of CRC cells. All experiments were repeated for three times, data were shown as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 in one-way ANOVA (g-i)

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Molecular Cancer

ISSN: 1476-4598