Fig. 6

circLONP2 and DDX1 collaboratively modulate pri-miR-17 processing. a Venn diagram showed potential common downstream miRNA targets of circLONP2 and DDX1 according to miRNA array results. b, c Verification of pri-miR-17 as a downstream target of circLONP2 by RT-qPCR, normalized to GAPDH. d, e circRIP and affinity pulldown revealed direct interaction of circLONP2 and pri-miR-17 through specific reverse complementary sequences, respectively, normalized to GAPDH. f RIP followed by RT-qPCR indicated circLONP2 mediated the interaction between pri-miR-17 and DDX1 or DGCR8. g, h Rescue experiments indicated that miR-17 was essential for circLONP2-enhanced migrating ability of CRC cells. All experiments were repeated for three times, data were shown as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 in Mann-Whitney U test (b), one-way ANOVA (c-e, g-h), or independent Student’s t test (f)