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Fig. 3 | Molecular Cancer

Fig. 3

From: Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2′-O-methylation via NOP58 recruitment in colorectal cancer

Fig. 3

ZFAS1 inhibits RNA stability of SNORD12C, SNROD78, and the specific sites of rRNA 2′-O-methylation. a, Reducing NOP58, SNORD12C, and SNROD78 half-life (t1/2) by silencing ZFAS1 in SW620 cells. Values are the mean ± s.d. of n = 3 independent experiments. b, The rRNAs 2′-O-Me activities of G3878 site and G4593 site were decreased after ZFAS1 knockdown in SW620 and HCT116 detected by RTL-P assay. c, The schematic structures showing a novel method called double-stranded primer based on single-stranded toehold (DPBST) assay for detecting rRNAs 2′-O-Me levels. d and e, The 28S rRNA G3878 and G4593 sites of 2′-O-Me mediated by SNORD12C or SNORD78 at the high or low dNTPs conditions after silencing ZFAS1 (Upper) or overexpression ZFAS1 (Lower) in SW620 cells detected by DPBST assays. f, The DPBST detecting statistical results of 2′-O-Me by qPCR assay. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

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