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Fig. 4 | Molecular Cancer

Fig. 4

From: Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2′-O-methylation via NOP58 recruitment in colorectal cancer

Fig. 4

Effects of ZFAS1 on cell proliferation and apoptosis by regulating NOP58 expression in CRC cells. a, CCK8 assays were used to identify the cell proliferation abilities upon ZFAS1 silencing or overexpressing in HCT116 and SW620 cells. n = 3 independent experiments. b, The migrated cell numbers were determined after ectopic or knockdown ZFAS1 in HCT116 and SW620 cells. c, The percentage (%) of cell apoptosis was detected upon ZFAS1 overexpressing or silencing in HCT116 and SW620 cells by Flow cytometry. n = 3 independent experiments. d and e, The NOP58 expression was conducted after overexpressing or knocking down ZFAS1 by Western blot (d), and IF assays (e). f, ISH and IHC methods were used to determine the cell localization and expression levels of ZFAS1 and NOP58 in CRC tissues and matched adjacent-tumor controls (n = 157). Scale bars = 100 μm. g, Violin charts displaying the expression scores and levels of ZFAS1 and NOP58 between CRC tissues and matched paired adjacent normal tissues (n = 157). Nonparametric tests and Median and 95%CI were shown. h, Kaplan-Meier curves representing the impact of ZFAS1 and NOP58 on overall survival in this included CRC cohort.*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

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