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Fig. 6 | Molecular Cancer

Fig. 6

From: Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2′-O-methylation via NOP58 recruitment in colorectal cancer

Fig. 6

SNORD12C/78-mediated 2′-O-Me modification regulates target genes expression in a ZFAS1-dependent manner. a and b, EIF4A3 and LAMC2 mRNA expression was determined after knocking down SNORD12C or SNORD78 by qRT-PCR assays(a), and Western blot method(b). c, Reducing EIF4A3 and LAMC2 half-life by silencing SNORD12C treated by the ActD in SW620 cells. d, Reducing the translation activity of EIF4A3 and LAMC2 by silencing SNORD12C and SNORD78 treated by the CHX in SW620 cells. e, The 28S rRNA G3878 and G4593 2′-O-Me activities were declined after silencing SNORD12C or SNORD78 expression in SW620 and HCT116 cells detected by RTL-P assays. f, Overexpressing ZFAS1 rescued the 2′-O-Me activities under the lower dNTPs conditions after silencing SNORD12C and SNORD78. g, The statistical plot of overexpressing ZFAS1 rescued the 2′-O-Me activities after silencing SNORD12C and SNORD78 *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

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