Fig. 3

circCCDC9 suppresses GC cells proliferation, migration and invasion in vitro. a. The schematic illustration of small interfering RNAs (siRNAs) and circCCDC9 expression vector specifically targeting the backsplice junction sequences. b. qRT-PCR analysis of circCCDC9 and CCDC9 mRNA in MKN-45 cells treated with siRNAs. c. qRT-PCR analysis of circCCDC9 and CCDC9 mRNA in AGS cells stably overexpressing circCCDC9. d-f. CCK-8 assays, colony formation assays and EdU assays were performed to determine the ability of proliferation in MKN45 cells transfected with si-circ or NC and AGS cells transfected with oe-circ or vector. Scale bar, 100 μm. g and h. Cell migratory and invasive capabilities were assessed by wound healing assays and transwell assays in MKN45 cells transfected with si-circ or NC and AGS cells transfected with oe-circ or vector. The white scale bar indicated 20 μm; the black scale bar indicated 200 μm. Data were showed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001