Fig. 5

MiR-6792-3p promotes GC cells proliferation, migration and invasion in vitro by targeting CAV1. a. Schematic illustration exhibiting overlapping of the target genes of miR-6792-3p predicted by circNET, miRTarBase, RNA22 and TargetScan. b. Relative mRNA and protein levels of CAV1 were evaluated by qRT-PCR (left) and western blot (right) in cells transfected with the miR-6792-3p mimics or inhibitor, respectively. c. Schematic illustration of CAV1-WT and CAV1-MUT luciferase reporter vectors. d. The relative luciferase activities were detected in MKN-45 and AGS cells after co-transfection with CAV1-WT or CAV1-MUT and mimics, inhibitor or NC, respectively. e-g. CCK-8 assays, colony formation assays and EdU assays were performed to determine the ability of proliferation in MKN45 cells transfected with mimics or mimics NC and AGS cells transfected with inhibitor or inhibitor NC. Scale bar, 100 μm. h and i. Cell migratory and invasive capabilities were assessed by wound healing assays and transwell assays in MKN45 cells transfected with mimics or mimics NC and AGS cells transfected with inhibitor or inhibitor NC. The white scale bar indicated 20 μm; the black scale bar indicated 200 μm. Data were showed as mean ± SD; ns indicated no significance.*P < 0.05, **P < 0.01, ***P < 0.001