Fig. 3

CircNFIB1 suppresses lymphangiogenesis in vitro. a-d qRT-PCR analysis of circNFIB1 and NFIB expression following circNFIB1-silencing (a and b), circNFIB1 overexpression (c and d) or corresponding control PDAC cells. e-j Representative images (e and h) and histogram analysis of tube formation (f and i) and Transwell (g and j) assays by HLECs treated with conditioned medium from circNFIB1-silencing or control PDAC cells. Scale bar: 100 μm. k-p Representative images (k and n) and histogram analysis of tube formation (l and o) and Transwell (m and p) assays by HLECs treated with conditioned medium from circNFIB1-overexpressing or control PDAC cells. Scale bar: 100 μm. Significance level was assessed using two-tailed Student t-tests and one-way ANOVA followed by Dunnett’s tests for multiple comparisons. Figures with error bars showed the standard deviations of three independent experiments. *p < 0.05 and **p < 0.01