Fig. 4

CircNFIB1 inhibits LN metastasis of PDAC in vivo. a and b Representative images (a) of bioluminescence and histogram analysis (b) of popliteal LN metastasis after silencing circNFIB1 (n = 12 per group). c Representative images for the nude mouse popliteal LN metastasis model. PDAC cells were injected into the footpads of the nude mice and the popliteal LNs were shown. d Representative images of enucleated popliteal LNs (n = 12 per group). e Histogram analysis of the LN volume in the indicated groups. f The ratio of popliteal LN metastasis was calculated for all groups (n = 12 per group). Significance level was assessed using two-tailed Student t-tests and one-way ANOVA followed by Dunnett’s tests for multiple comparisons. Figures with error bars showed the standard deviations of three independent experiments. *p < 0.05 and **p < 0.01