Fig. 8

CircNFIB1 inhibits lymphangiogenesis and LN metastasis of PDAC via VEGF-C suppression. a-f Representative images (a and d) and a histogram analysis of tube formation (b and e) and Transwell (c and f) assay by HLECs treated with conditioned medium from si-NC, si-circNFIB1, si-circNFIB1 + PBS, and si-circNFIB1 + αVEGF-C PDAC cells. Scale bar: 100 μm. g Histogram analysis of the LN volume in the indicated groups. h The ratio of popliteal LN metastasis was calculated for all groups (n = 12 per group). i Kaplan-Meier survival curves for indicated groups (n = 12 per group). Significance level was assessed using two-tailed Student t-tests and one-way ANOVA followed by Dunnett’s tests for multiple comparisons. Figures with error bars showed the standard deviations of three independent experiments. *p < 0.05 and **p < 0.01