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Fig. 3 | Molecular Cancer

Fig. 3

From: RETRACTED ARTICLE: LncRNA BCRT1 promotes breast cancer progression by targeting miR-1303/PTBP3 axis

Fig. 3

LncRNA BCRT1 knockdown inhibited breast cancer cell metastasis in vitro and in vivo. a Transwell migration and invasion assays were used to evaluate the motility of MDA-MB-231 and MDA-MB-468 cells transfected with si-NC or si-BCRT1. Columns are the average of three independent experiments. b LncRNA BCRT1 overexpression led to increased migration and invasion of MDA-MB-231 and MDA-MB-468 cells. c Tube formation in HUVECs was inhibited by conditioned medium from MDA-MB-231 cells transfected with si-BCRT1 and was promoted by that from MDA-MB-231 cells transfected with the lncRNA BCRT1-overexpressing vector. d EMT-related proteins were detected by western blot in MDA-MB-231 and MDA-MB-468 cells after knockdown or overexpression of lncRNA BCRT1. e Stably transfected MDA-MB-231 cells were injected into the tail veins of nude mice (n = 5). Representative images of lungs and H&E staining of lungs isolated from mice. LncRNA BCRT1 overexpression resulted in an increased number of lung metastatic colonies. f Representative immunohistochemistry staining of CD31 in the indicated xenografts. The corresponding statistical plots are presented in the lower panel. (**P < 0.01 and ***P < 0.001)

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