Fig. 6

LncRNA BCRT1 could be secreted by breast cancer cells and promoted M2 polarization. a RT-PCR was used to detect the expression of M1 markers and M2 markers after LPS/INF-γ or IL-4/IL-13 treatment. b The expression of lncRNA BCRT1 was elevated in M2 macrophages. c M1 markers (CD80, MCP-1, iNOS, and IL-6) were significantly increased, while M2 markers (CD206 and MRC-2) were remarkably decreased in the lncRNA BCRT1 knockdown group. d LncRNA BCRT1 overexpression led to decreased expression of M1 markers and increased expression of M2 markers. e Conditioned medium derived from lncRNA BCRT1-overexpressing cells further increased the expression of M2 markers and lncRNA BCRT1 in macrophages. f Western blotting analysis of the exosomal markers CD63, Hsp70 and Hsp90 in exosomes derived from breast cancer cells with or without lncRNA BCRT1 overexpression. g Agarose gel electrophoresis and RT-PCR assays were used to detect the expression of lncRNA BCRT1 in exosomes. h Representative microscopy showing the uptake of PKH26-labeled exosomes (red fluorescent dye) derived from MDA-MB-231 cells by recipient macrophages. i The expression of M2 markers and lncRNA BCRT1 in macrophages was detected after culture with the indicated exosomes. j Cell migration was increased after cultured with lncRNA BCRT1-overexpressing exosomes or conditioned media. k-l Tube formation or CAM assays were used to evaluate the angiogenesis ability after culture with lncRNA BCRT1-overexpressing exosomes or conditioned media. (*P < 0.05, **P < 0.01, and ***P < 0.001)