Fig. 8
From: RETRACTED ARTICLE: LncRNA BCRT1 promotes breast cancer progression by targeting miR-1303/PTBP3 axis
LncRNA BCRT1 is essential for HIF-1α-mediated hypoxia-induced malignant properties. MDA-MB-231 cells were treated with normoxia, hypoxia, a combination of siHIF-1α and hypoxia, a combination of si-BCRT1 and hypoxia, or hypoxia and cotransfection with siHIF-1α and the pcDNA3.1-BCRT1 plasmid. a The expression of HIF-1α and PTBP3 was assessed by western blot. b The expression of lncRNA BCRT1 and PTBP3 was evaluated by RT-PCR. c Colony formation assay was used to evaluated the proliferation of MDA-MB-231 cells. d Monolayer morphology of MDA-MB-231 cells were photographed. e-f Transwell assay and wound healing assay were used to analyze the migration ability of MDA-MB-231 cells. (*P < 0.05, **P < 0.01, and ***P < 0.001)