Fig. 2

CircSHKBP1 promotes GC cell proliferation, migration and invasion in vitro. a and b Level of circSHKBP1 in BGC823, HGC27, AGS, MGC803 and GES1 cells and their medium-derived exosomes, as determined by qRT-PCR (normalized to GES1 levels). c and d Identification of medium exosomes of BGC823 cells by TEM and western blot. e Assessment of the proliferation of BGC823 cells transfected with control or circSHKBP1 siRNAs by CCK8 assay. f and i Assessment of the proliferation of BGC823 cells transfected with control or circSHKBP1 siRNAs by EdU assay. g Assessment of the proliferation of BGC823 cells transfected with control vector or circSHKBP1 plasmid by CCK8 assay. h and l Assessment of the proliferation of BGC823 cells transfected with control vector or circSHKBP1 plasmid by EdU assay. j and k Assessment of the migration and invasion of BGC823 cells transfected with control or circSHKBP1 siRNAs by Transwell assay. m and n Assessment of the migration and invasion of BGC823 cells transfected with control vector or circSHKBP1 plasmid by Transwell assay. o Assessment of the proliferation of BGC823 cells cocultured with circSHKBP1-overexpressing exosomes or normal exosomes for 48 h at different concentrations by CCK8 assay. p and q Assessment of the migration of BGC823 cells cocultured with circSHKBP1-overexpressing exosomes or normal exosomes by Transwell assay. Quantitative data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test)