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Fig. 4 | Molecular Cancer

Fig. 4

From: Circular RNA circMET drives immunosuppression and anti-PD1 therapy resistance in hepatocellular carcinoma via the miR-30-5p/snail/DPP4 axis

Fig. 4

circMET may function as a sponge for miR-30-5p a. The circRIP assay showed a specific enrichment of circMET and miR-30-5p compared with that in the negative control; b. A schematic drawing showing the putative binding sites of wt-miR-30s-5p and mu-miR-30s-5p in circMET; c. The luciferase activity of luc-circMET and mutant luc-circMET in HEK293T cells after cotransfection with miR-30-5p; d. The circRIP assay was performed in HEK293T cells using biotin-labeled miR-30-5p mimics and a negative control (NC); e. FISH analysis in HCCLM3 and Huh7 cells revealed that circMET colocalized with miR-30a-5p in the cytoplasm; f. miR-30a/e-5p knockdown in cells with low levels of circMET upregulated the cell invasive ability. Left, representative pictures of transwell assay. Right, statistic of transwell assay

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