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Fig. 5 | Molecular Cancer

Fig. 5

From: Circular RNA circMET drives immunosuppression and anti-PD1 therapy resistance in hepatocellular carcinoma via the miR-30-5p/snail/DPP4 axis

Fig. 5

circMET induces immunosuppression in HCC through the miR-30-5p/Snail/DPP4 axis a. A schematic drawing showing the putative binding sites of human Snail and miR-30a-5p; b. The luciferase activity of luc-Snail in HEK293T cells after cotransfection with miR-30a-5p; c. The expression of Snail in HCC cells with different circMET and miR-30-5p expression levels; d. The overlap of RNA-seq data from cells with different circMET levels and Snail Chip-seq data, followed by overlap with the immune related genes from RNA-seq; e. The binding site of Snail with the genes CSPP1, PDGFB and DPP4 according to the Snail Chip-seq results; f. Diagrammatic sketch of the Snail-NC, Snail-OE, DPP4-promoter-Luc-NC and DPP4-promoter-Luc-OE vectors; g. The luciferase activity of the DPP4 reporter was upregulated in cells cotransfected with Snail DPP4-promoter and 21,975 + TK/21976 + TK; h. The DPP4 mRNA level is related to the level of Snail in HCC cells; i. The DPP4 protein level is related to the level of Snail in HCC cells; j. DPP4 levels in the supernatant of HCC cells with different levels of circMET; k and l. A positive relationship between Snail and DPP4, and a negative relationship between DPP4 and CD8+ T cells were observed in HCC tissues

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