Fig. 5

LINC00173.v1 functions as a competitive endogenous RNA for miR-511-5p. a Volcano plot analyzed the clinical correlation of LINC00173.v1 with all reported miRNAs in SQC dataset from TCGA (n = 502). The red colors represent significantly and negatively correlated miRNAs with fold change < 0.5 and r value < − 0.1. b Real-time PCR analysis of the effect of LINC00173.v1 on multiple miRNAs expression in the indicated cells. Pseudo-color scale values were log2 transformed. Transcript levels were normalized by U6 expression. The experiment was independently performed three times. c Potential binding sites of mutant and wild-type LINC00173.v1 on miR-511-5p. d Real-time PCR analysis of miR-511-5p expression compared with mutant and wild-type LINC00173.v1. Transcript levels were normalized by U6 expression. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by one-way ANOVA test. e Luciferase reporter activity of the influence of miR-511-5p on LINC00173.v1. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by one-way ANOVA test. f Effect of miR-511-5p on human umbilical vein endothelial cells (HUVECs) in tube formation assay in the indicated lung cancer cells. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test. g Effect of miR-511-5p on length of the 2nd and 3rd vessels on chick embryo chorioallantoic membrane (CAM) in the indicated lung cancer cells. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test. h Influence of miR-511-5p on secretion level of VEGF-A in the indicated lung cancer cells. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test