Fig. 7
Squamous cell carcinoma-specific factor ΔNp63α contributes to LINC00173.v1 overexpression in SQC. a LINC00173.v1 expression levels in lung cancer tissues with low (n = 168), middle (n = 168) and high (n = 166) ΔNp63α expression from TCGA SQC dataset. Each bar represents the median values ± quartile values. P value was determined by one-way ANOVA test. b Positive percentage of ΔNp63α staining in 43 benign lung granuloma, 248 SQC tissues, 122 ADC tissues and 26 other subtypes of NSCLC. P value was determined by χ2 test. c Cases of sample of immunohistochemical staining of ΔNp63α in SQC tissues stratified by low (n = 93) and high (n = 155) LINC00173.v1 expression levels. P value was determined by Fisher’s test. d Real-time PCR analysis of LINC00173.v1 expression in lung cancer cell lines with changed expression levels of ΔNp63α. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test or one-way ANOVA test. e ΔNp63α-binding motifs in the putative promoter region of LINC00173.v1 in UCSC genome browser. f ChIP analysis of the binding sites of ΔNp63α in the promoter region of LINC00173.v1 in lung cancer cells. IgG was used as negative control. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test. g Luciferase reporter activity of LINC00173.v1 promoter in ΔNp63α-overexpressing lung cancer cells. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05 by unpaired t test. h Hypothetical model illustrating that squamous cell carcinoma-specific factor ΔNp63α contributes to LINC00173.v1 overexpression in SQC tissues, which further promotes proliferation and migration of vascular endothelial cells and progression of lung squamous cell carcinoma through sponging miR-511-5p to regulate VEGFA expression