Fig. 3

METTL14 inhibits CRC cell migration, invasion and metastasis. a. The expression of METTL14 in CRC cell lines (HCT116, HCT8, HT29, SW620, SW480 and DLD-1) compared with NCM460 detected using qRT-PCR. b. The protein levels of METTL14 in HCT116 and HCT8 cells with METTL14 knockdown or METTL14 overexpression were measured by western blot. c. Transwell migration assays were used to determine the migratoy abilities of HCT116 and HCT8 cells with METTL14 knockdown or overexpression. d. Transwell invasion assays were employed to detect the invasive abilities of HCT116 and HCT8 cells with METTL14 knockdown or overexpression. e. Left panel, representative images of metastatic nodes in the lungs from LV-shNC and LV-shME-1 groups. Middle panel, quantification of the metastatic nodes from LV-shNC and LV-shME-1 groups. Right panel, HE-stained lung sections from LV-shNC and LV-shME-1 groups. f. Left panel, representative images of metastatic nodes in the lungs from LV-NC and LV-METTL14 groups..Middle panel, quantification of the metastatic nodes from LV-NC and LV-METTL14 groups. Right panel, HE-stained lung sections from LV-NC and LV-METTL14 groups.**P < 0.01, ***P < 0.001