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Fig. 2 | Molecular Cancer

Fig. 2

From: Circular RNA circSATB2 promotes progression of non-small cell lung cancer cells

Fig. 2

circSATB2 directly binds to miR-326 and regulates miR-326 expression in NSCLC cells. a FISH for subcellular localization of circSATB2. 6-FAM labeled the circSATB2 FISH probe, DAPI stained cell nuclei. b Cytoplasmic and nuclear circSATB2 expression detected by qPCR. U6 and GAPDH acted as nuclear and cytoplasmic reference genes, respectively. Relative expression measured by the 2-(cytoplasmic Ct value - nuclear Ct value) method. c miRNA expression in BEAS-2B and NSCLC cells detected by qPCR. d Sequence alignments between circSATB2 and seed sequences of miRNAs. WT, wild-type vector; Mut, mutant sequence vector of circSATB2. eg Dual luciferase reporter gene assay to detect interaction between circSATB2 and miRNAs. Mimic NC, empty vector control; mimic, miRNA mimic. h miR-326 expression in circSATB2-sh and circSATB2-OE stably transfected lung cancer cells detected by qPCR. All experiments repeated independently three times. Data are presented as means±standard deviation. *P < 0.05, **P < 0.01 compared with BEAS-2B cells (3c), mimic NC group (3e-g), and lv3 group (3 h); ##P < 0.01 compared with lv5 group

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