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Fig. 3 | Molecular Cancer

Fig. 3

From: Circular RNA circSATB2 promotes progression of non-small cell lung cancer cells

Fig. 3

circSATB2 regulates FSCN1 expression via miR-326. acFSCN1 and GAB1 mRNA expression in circSATB2-knockdown and overexpressing stably transfected lung cancer cells detected by qRT-PCR. dfFSCN1 and GAB1 mRNA expression in miR-326-knockdown and overexpressing lung cancer cells detected by qRT-PCR. gFSCN1 mRNA expression in NSCLC cells after co-transfection with circSATB2 and miR-326 overexpression vectors. h Sequence alignments (upper) and interaction between miR-326 and FSCN1 detected by dual luciferase reporter gene assay (under). WT, wild-type vector; Mut, mutant sequence vector of FSCN1; mimic NC, empty vector control; mimic, miR-326 mimic. i, j Correlation between circSATB2 and FSCN1 mRNA expression in NSCLC cells. k, l FSCN1 protein expression in circSATB2-knockdown and overexpressing stably transfected H460, A549 and H1299 cells detected by western blot. m, n FSCN1 protein expression in miR-326-knockdown and overexpressing H460, A549 and H1299 cells detected by western blot. o, p FSCN1 protein expression in BEAS-2B, H460, A549 and H1299 cells detected by western blot. All experiments were independently repeated three times. Data are presented as means±standard deviation. **P < 0.01 compared with BEAS-2B, lv3, or inhibitor NC group; #P < 0.05, ##P < 0.01 compared with lv5 group

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