Fig. 5

circSATB2 affects cell proliferation, migration and invasion of NSCLC cells and increases proliferation of normal cells via exosomes. a Exosome identification by TEM. b Nanosight-detected size range of cellular exosome diameters. c Western blot detected expression of exosome marker proteins TSG101, CD9 and CD63. d circSATB2 expression in exosomes derived from normal bronchial epithelial cells and NSCLC cells detected by qPCR. e Exosomes from fluorescence-labeled cells were taken up by BEAS-2B and NSCLC cells. f circSATB2 expression after co-culture with circSATB2-OE and circSATB2-sh exosomes detected by qPCR. g, h EdU assay to detect proliferation of H460 and A549 cells after co-culture with circSATB2-sh and circSATB2-OE exosomes. i, k Wound healing assay to detect migration of H460 and A549 cells after co-culture with circSATB2-sh and circSATB2-OE exosomes. j, l Transwell migration assay to detect migration of H460 and A549 cells after co-culture with circSATB2-sh and circSATB2-OE exosomes. m, n Transwell invasion assay to detect invasion of H460 and A549 cells after co-culture with circSATB2-sh and circSATB2-OE exosomes. o CCK-8 assay to detect the cell viability of BEAS-2B cells after co-culture with circSATB2-sh and circSATB2-OE exosomes. p, q Colon formation assay to detect the proliferation of BEAS-2B cells after co-culture with circSATB2-sh and circSATB2-OE exosomes. All experiments were repeated independently three times. Data are presented as means±standard deviation. ^**P < 0.01 compared with BEAS-2B-exo or lv3-exo group; ^#P < 0.05, ^##P < 0.01 compared with lv5-exo group