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Fig. 4 | Molecular Cancer

Fig. 4

From: Cancer cell-derived exosomal circUHRF1 induces natural killer cell exhaustion and may cause resistance to anti-PD1 therapy in hepatocellular carcinoma

Fig. 4

CircUHRF1 inhibits the biological function of miR-449c-5p in NK cells. a RIP was performed for circRNA in NK-92 cells using a circUHRF1 probe and a negative control (NC) probe. b RIP experiments were carried out using an anti-AGO2 antibody with NK-92 cell extracts. c The level of circUHRF1 in the streptavidin-captured fractions of NK-92 cell lysates after transfection with biotinylated miR-449c-5p or the negative control (NC). CircANRIL was used as a negative control. d Putative binding sites of miR-449c-5p on circUHRF1 were predicted by StarBase v3.0. e The luciferase activity of pGL3-circUHRF1 in NK-92 cells after cotransfection with miR-449c-5p. f CircUHRF1 expression in NK-92 cells was modified by lentivirus-mediated overexpression and knockdown. g The relative level of miR-449c-5p was measured by qRT-PCR in NK-92 cells transfected with circUHRF1, shcircUHRF1, mock, or the negative control. The data are presented as the mean ± SD; n = 3, **P < 0.01, ***P < 0.001, NS: not significant

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