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Fig. 5 | Molecular Cancer

Fig. 5

From: Cancer cell-derived exosomal circUHRF1 induces natural killer cell exhaustion and may cause resistance to anti-PD1 therapy in hepatocellular carcinoma

Fig. 5

CircUHRF1 regulates the miR-449c-5p/ TIM-3 pathway in NK cells. a The putative binding site of miR-449c-5p on TIM-3 was predicted by StarBase v3.0. b Luciferase activity of pGL3-TIM-3 in NK-92 cells cotransfected with miR-449c-5p. c and d The levels of TIM-3 mRNA and protein were measured by qRT-PCR and western blotting, respectively, in NK-92 cells transfected with miR-449c-5p or miR-449c-5p combined with circUHRF1. e A negative correlation between circUHRF1 and miR-449c-5p expression was observed in NK cells from HCC patients (R2 = 0.3065; P = 0.0002). f A positive correlation between circUHRF1 and TIM-3 mRNA expression was observed in NK cells from HCC patients (R2 = 0.4650; P < 0.0001). g A negative correlation between miR-449c-5p and TIM-3 mRNA expression was observed in NK cells from HCC patients (R2 = 0.1166; P = 0.0310). The data are presented as the mean ± SD; n = 3, *P < 0.05, **P < 0.01, NS: not significant

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Molecular Cancer

ISSN: 1476-4598