Fig. 2

BATF2 inhibits GC growth in vitro and in vivo. a HGC-27 cells with stable BATF2 overexpression or SNU-216 cells with BATF2 knockdown were created. The changes in BATF2 expression were confirmed using western blotting. b-e The proliferative ability of stably transfected HGC-27 or SNU-216 cells was investigated via colony formation and CCK-8 assays (**P < 0.01). f-g Flow cytometry analysis of stably transfected HGC-27 or SNU-216 cells was performed. Representative images and quantification of the results are presented (**P < 0.01; ***P < 0.001). h Overexpression of BATF2 inhibits GC growth in a subcutaneous xenograft model. The size of the tumors was measured at the indicated time points (***P < 0.001). Tumors were extracted and weighed after mice were sacrificed (***P < 0.001). i BATF2 knockdown promotes GC growth in a subcutaneous xenograft model. The size of the tumors was measured at the indicated time points (***P < 0.001). Tumors were extracted and weighed after mice were sacrificed (***P < 0.001)