Fig. 3

BATF2 suppresses GC metastasis in vitro and in vivo. a-b Transwell assays with stably transfected HGC-27 and SNU-216 cells were performed. Representative images and quantification of the results are presented (*P < 0.05; **P < 0.01; ***P < 0.001). c Representative bioluminescence images of mice at 4 weeks after tail vein injection of BATF2-overexpressing HGC-27 cells or control cells and quantification of the images are presented. d-e Representative images of lung metastasis and H&E (hematoxylin and eosin) staining are shown. Metastatic nodules were counted with or without a microscope and recorded. Overexpression of BATF2 in HGC-27 cells significantly reduced the number of metastatic lesions in the lungs (***P < 0.001). Scale bars = 200 μm. f Stably transfected HGC-27 cells were injected intraperitoneally, and the number of metastases in the colonic wall was recorded 4 weeks later. Peritoneal metastases were examined and recorded (**P < 0.01)