Fig. 5

CBX4 is a direct target of miR-136-5p. a Schematic illustration exhibiting the overlap between the target mRNAs and miR-136-5p as predicted by TargetScan, miRDB, Starbase and miRTarBase. b Comprehensive transcriptional analysis indicated that miR-136-5p expression was negatively correlated with CBX4 expression in RCC tissues. c Schematic illustration of CBX4-WT and CBX4 Mut luciferase reporter vectors. d Relative luciferase activities were measured in RCC cells after transfection with CBX4-WT or CBX4 Mut and a miR-136-5p mimic or miR-NC. e CBX4 mRNA expression was evaluated by performing qRT-PCR assays in RCC cells transfected with a miR-136-5p mimic. f and g CBX4 protein expression was detected by performing western blot and immunofluorescence assays in RCC cells transfected with a miR-136-5p mimic. h CBX4 expression was increased in RCC tissues compared to matched normal tissues. i CBX4 expression in RCC patients with different tumor sizes. j CBX4 expression in RCC patients with no metastasis or distant metastasis. k Kaplan-Meier analysis of the overall survival of RCC patients with high and low expression of CBX4. *p < 0.05, **p < 0.01, no significant (NS)