Fig. 1

Candidate microRNAs that may be influenced by Bmi-1 expression. a Hierarchical clustering of microarray data showing the comparison of miRNA expression profiles between the two groups (GES-1-Bmi-1 cells and their control). b RKIP was predicted as a target of these differentially upregulated miRNAs, and their predicted binding site in the RKIP 3’UTR is shown. c qRT-PCR analysis of differentially expressed miR-27a and miR-155 in GES-Bmi-1 cells. *P < 0.05 vs. GES-1-Vector. d The gene expression levels of miR-155 and miR-27a were downregulated by siBmi-1 GC cells. **P < 0.01 vs. siNC. e qRT-PCR analysis of miR-27a and miR-155 expression in 15 paired GC tissues (T) and adjacent normal tissue samples (N). *P < 0.05. f Kaplan-Meier showed the correlations between miR-155 and miR-27a and the 3-year overall survival of patients with intestinal-type or diffuse-type GC from TCGA