Fig. 1

LncRNA RBAT1 is highly expressed in Retinoblastoma and Bladder Cancer cells. a Volcano plots of differentially expressed lncRNAs. The X-axis represents log fold changes. The Y-axis represents log p values. The blue points denote the significantly downregulated lncRNAs, and the red points denote the significantly upregulated lncRNAs. b RNA-sequence analysis was performed to evaluate the transcriptome in three retinoblastoma samples and three adjacent retina samples. Schematic annotation of the E2F3 genomic locus on Chr6p22.3; the gray rectangles represent the exons of E2F3, and the green rectangles represent RBAT1. c Real-time PCR analysis of RBAT1 expression in different tumor cell lines. *p < 0.05 and **p < 0.01. d and e RBAT1 expression levels in Rb tissues (d) and BCa tissues (e). RBAT1 was highly expressed in Rb and BCa tissues compared with normal tissues. **P < 0.01. f RNA FISH analysis shows that RBAT1 was located predominantly in the nucleus of Rb and BCa cell lines. g Cell nuclear/cytoplasmic fraction analysis and real-time PCR confirmed that RBAT1 was expressed mainly in the nucleus; GAPDH and U6 RNA served as positive controls for the cytoplasmic and nuclear fractions, respectively