Skip to main content
Fig. 3 | Molecular Cancer

Fig. 3

From: A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3

Fig. 3

RBAT1 activates E2F3 signaling. a Gene ontology (GO) analysis was conducted to identify enriched biological processes in WERI-Rb-1. b A heat map shows the mRNA expression levels of several genes in RBAT1-silenced or Ctrl-treated WERI-Rb-1 cells and 5637 cells. CDKAL1 and MBOAT1 are the genes near E2F3, and the genes in red box are targets of E2F3. c An interaction analysis of the altered target genes in E2F3 signaling. d-f E2F3 and some of its downstream genes in the microarray were verified in Y79, WERI-Rb-1 and 5637 cells by real-time PCR. The results are shown as mean ± SD in three independent experiments. *p < 0.05. g shRNA knockdown efficacies were validated by western blot, and E2F3 protein levels were decreased after RBAT1 silencing by GapmeRs. h CDC6 and CCNA1 were analyzed by western blotting

Back to article page

Molecular Cancer

ISSN: 1476-4598