Fig. 5

circPACRGL promotes CRC proliferation, migration, and invasion by regulating miR-142-3p/miR-506-3p-TGF-β1 axis. a Cell proliferation was determined by CCK8 assay. HCT116 cells were stimulated by CRC cell-derived exosomes or not, along with circPACRGLsiRNA transfection, and with or without miR-142-3p/miR-506-3p inhibitor (inh) or pcDNA3.1-TGF-β1 transfection, and detected at 0, 24, 48 and 72 h after treatment. b The cell apoptosis assay was determined by Annexin V/PI staining using flow cytometry analysis after the indicated treatment. c Representative micrographs of the wound healing assay. After the indicated treatment, images of HCT116 cells were taken at 0 h and 24 h after scratching. d Cell migration and invasion assays using Transwell or Matrigel-coated Transwell in HCT116 and SW480 cells after the indicated treatment. All P values were determined by a two-tailed unpaired student’s t-test (**, P < 0.01, compared to the control group; ^^, P < 0.01, compared to the Control + circPACRGLsiRNA pool group; ##, P < 0.01, compared to the Ex-HCT116 + circPACRGLsiRNA pool group). Ex, exosomes