Fig. 6
Cancer-derived exosomal circPACRGL regulates differentiation of N1-N2 neutrophils via miR-142-3p/miR-506-3p-TGF-β1 axis in murine colon cancer cells (CT-26). The cell apoptosis assay was determined by Annexin V/PI staining using flow cytometry analysis after the indicated treatment. CD11b + Ly6G + Ly6C+ is the marker of N1 neutrophils; CD11b + Ly6G + Ly6Clow is the marker of N2 neutrophils. All P values were determined by a two-tailed unpaired student’s t-test (**, P < 0.01, compared to the control group; ^^, P < 0.01, compared to the Control+circPACRGLsiRNA pool group; ##, P < 0.01, compared to the Ex-CT-26 + circPACRGLsiRNA pool group). Ex, exosomes