Fig. 7

Dysregulation of the ALKBH5-LYPD1 axis triggers HCC malignancy. a and b CCK-8 proliferation assays were conducted in either ALKBH5-knockdown or LYPD1-knockdown Huh7 (a) and MHCC97H (b) cells; c and d Colony formation assays were carried out in either ALKBH5-silenced or LYPD1-silenced Huh7 (c) and MHCC97H (d) cells. Column diagrams (right panel) showed colony numbers of each group; e, f and g Representative images of transwell assays to examine the effects of LYPD1 knockdown on ALKBH5-silenced Huh7 (e) and MHCC97H (f) cells were shown (scale bars, 200 μm); Quantification data presented the relative count (refer to negative control group) of cells which passed through the chamber membrane (g); h Representative images of wound healing assays conducted in ALKBH5/LYPD1-rescued cells were shown (left panel). And percent area of wound healed in each group was quantified (right panel)