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Fig. 2 | Molecular Cancer

Fig. 2

From: β-catenin represses miR455-3p to stimulate m6A modification of HSF1 mRNA and promote its translation in colorectal cancer

Fig. 2

β-catenin stimulates HSF1 protein translation. a The effect of Pyrvinium on the protein expression of HSF1 was explored by western blotting. b The effect of β-catenin knockdown on HSF1 protein level was analyzed by western blotting. c The protein level of HSF1 before and after β-catenin overexpression was analyzed by western blotting. d The effect of LiCl on HSF1 protein level in RKO and MEF was analyzed by western blotting. e The expression of β-catenin and HSF1 in colorectal tissue was analyzed by immunohistochemistry staining. f The correlation between β-catenin expression and HSF1 expression in colorectal tissue was analyzed by chi-square test (*p < 0.01). g The effect of β-catenin depletion on HSF1 with puromycin labeling was determined by western blotting. h Amount of HSF1 mRNA in various polysome fractions was analyzed by RT-PCR(*p < 0.05)

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