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Fig. 4 | Molecular Cancer

Fig. 4

From: β-catenin represses miR455-3p to stimulate m6A modification of HSF1 mRNA and promote its translation in colorectal cancer

Fig. 4

m6A modification of HSF1 mRNA stimulates its protein translation. a The sites of HSF1 3′-UTR binding to the seed sequence of miR455-3p was consistent with m6A RNA modification elements “DRACH”. b Bioinformatic prediction of m6A modification in 3′-UTR of HSF1 mRNA. c m6A modification of HSF1 mRNA was analyzed by meRIP (*p < 0.05). d Binding of METTL3 to HSF1 mRNA was detected by RIP (*p < 0.05). e m6A modification of HSF1 mRNA with or without METTL3 depletion was analyzed by meRIP (*p < 0.05). f The protein level of HSF1 before and after METTL3 depletion was detected by western blotting. g The effect of METTL3 knockdown on HSF1 synthesis was determined by western blotting after puromycin labeling. h Amount of HSF1 mRNA in various polysome fractions was analyzed by RT-PCR(*p < 0.05). i The effect of YTHDF1 knockdown on HSF1 protein level was analyzed by western blotting

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Molecular Cancer

ISSN: 1476-4598