Fig. 5
β-catenin suppresses miR455-3p to increase HSF1 mRNA m6A modification. a m6A modification and METTL3 interaction of HSF1 mRNA with WT or mutant of miR455-3p were analyzed by RIP. b The interaction between biotin-miR455-3p and HSF1 mRNA with or without METTL3 depletion was analyzed by biotin pull down. c The effect of miR455-3p inhibitor and/or β-catenin knockdown on m6A modification of HSF1 mRNA was analyzed by meRIP. d The effect of miR455-3p inhibitor and/or β-catenin knockdown on the interaction of METTL3 with HSF1 mRNA was analyzed by RIP. e The effect of β-catenin knockdown on interaction of miR455-3p and HSF1 mRNA was analyzed by biotin pull down. f and g The levels of mature (f) and primary (g) miR455-3p with β-catenin knockdown or LiCl treatment were determined by RT-PCR. h The correlation of COL27A1 and miR455 was analyzed in linkedomics (http://linkedomics.org/). i The effect of β-catenin depletion or LiCl on mRNA level of COL27A1 was analyzed by RT-PCR. j The interaction of β-catenin/TCF7L2 and HSF1 promoter was determined by ChIP. k The correlation of β-catenin protein expression with the RNA level of COL27A1 was detected by linkedomics (http://linkedomics.org/)