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Fig. 6 | Molecular Cancer

Fig. 6

From: Identification of miPEP133 as a novel tumor-suppressor microprotein encoded by miR-34a pri-miRNA

Fig. 6

miPEP133 has p53-independent functions. a Representative western blot images of miPEP133 in ovarian cancer cell line SKOV3 and cervical cancer cell line Hela. GAPDH was used as loading control. b Representative western blot images of miPEP133 in control and miPEP133-overexpressing SKOV3 and Hela cells. c RT-QPCR of miPEP133 in control and miPEP133-overexpressing SKOV3 and Hela cells. Student’s t-test, *p < 0.01, #p < 0.0001. d RT-QPCR of miR-34a in control and miPEP133-overexpressing SKOV3 and Hela cells. Student’s t-test, *p < 0.01. e Cell growth rates of SKOV3 and Hela cells. Two-way ANOVA followed by Sidak’s test, *p < 0.05, **p < 0.005, ***p < 0.001, #p < 0.0001. f Flow cytometry dot plots of SKOV3 and Hela cells transfected with control or miPEPqee plasmid and stained with AnnexinV/7-ADD. g Summarized flow cytometry result of JC-1-staining in Hela cells transfected with control or miPEP133 plasmid. CCCP-treated Hela cells were used as positive control. Student’s t-test, **p < 0.005. h Representative western blot images of miPEP133 in patient-derived ovarian cancer cell lines, OVC201, OVC203, OVC205 and OVC303. Normal human fallopian tube epithelial cell lines, FT240 and FT246, were used as noncancerous control cells. GAPDH was used as loading control. i Representative western blot images of miPEP133 in ovarian cancer samples (T1-T8. Human normal uterus tissue protein lysate was used as a positive control in western blot. β-actin was used as loading control

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