Fig. 6

PERP is involved in the METTL14-induced Pancreatic Cancer Cells’ Growth and Invasion. a Viability of PANC-1 cells with or without PERP knockdown in the absence or presence of METTL14 knockdown analyzed by the CCK8 assay. ***p < 0.001. b Representative images from the colony-forming assay (lower panel) and colony number analysis (upper panel) as indicated. All experiments were performed in triplicate and data are presented as the mean ± SD. **, p < 0.01; ***, p < 0.001. c PANC-1 cells with or without PERP knockdown in the absence or presence of METTL14 knockdown were analyzed in a transwell assay with Matrigel. All experiments were performed in triplicate and data are presented as the mean ± SD. Scale bar: 200 μm. ** p < 0.01; ***p < 0.001. d Western blotting of PERP and Flag in PANC-1 cells with or without PERP WT transfection in the absence or presence of METTL14 overexpression (left panel); western blotting of PERP and Flag in PANC-1 cells with or without PERP 3′-UTR transfection in the absence or presence of METTL14 overexpression (right panel). e Colony-forming assay in PANC-1 cells with or without PERP WT transfection in the absence or presence of METTL14 overexpression (left panel); colony-forming assay in PANC-1 cells with or without PERP 3′-UTR transfection in the absence or presence of METTL14 overexpression (right panel). ** p < 0.01; ***p < 0.001; n.s., no significance. f Transwell assay in PANC-1 cells with or without PERP WT transfection in the absence or presence of METTL14 overexpression (upper panel); transwell assay in PANC-1 cells with or without PERP 3′-UTR transfection in the absence or presence of METTL14 overexpression (lower panel). Scale bar: 200 μm. ** p < 0.01; ***p < 0.001; n.s., no significance