Fig. 2

Effects of NPTXR knockout on survival phenotypes of GC cell lines. a Direct sequence analysis confirming successful editing of NPTXR exon 1. Insertion of a T around the target site of the gRNA was observed. Red arrow indicates the frame-shift site. b Western blot analysis confirming NPTXR knockout (KO) in MKN1 cells. c-g CCK-8 proliferation assay c, annexin V flow cytometric apoptosis assay d, flow cytometric mitochondrial membrane potential assay e, colorimetric activated caspase-3 and -9 assay f, and flow cytometric cell cycle analysis assay g of parental and NPTXR KO MKN1 cells. *P < 0.05. Mean ± standard deviation